SNAP25 antibody (pSer187)

Catalog No. ABIN5541045

The Rabbit Polyclonal anti-SNAP25 antibody has been validated for WB. It is suitable to detect SNAP25 in samples from Human, Rat, Mouse, Zebrafish (Danio rerio), Guinea Pig and Primate.

Quick Overview for SNAP25 antibody (pSer187) (ABIN5541045)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Guinea Pig, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SNAP25 Antibody

Binding Specificity: pSer187

Specificity: Specific for the ~25k SNAP25 protein phosphorylated at Ser 187 in Western blots. Immunolabeling is completely blocked by blocked λ -Ptase.

Purification: Affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser187 of SNAP25

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1/1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50 % glycerol

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: snap25

Background: SNAP25 (Synaptosomal associated pr otein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer's Disease (Greber et al., 1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al ., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

UniProt: P60881

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport