COL6 antibody

Catalog No. ABIN5541083

This Rabbit Polyclonal antibody specifically detects COL6 in IHC (p), IHC (fro), IF, EIA, ICC and RIA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Chicken and Pig.

Quick Overview for COL6 antibody (ABIN5541083)

Target: COL6 (Collagen, Type VI (COL6))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL6 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Product Details anti-COL6 Antibody

Specificity: Human Collagen type VI: 100 % binding in RIA at 1/10,000 dilution.

Purification: Affinity Chromatography.

Immunogen: Purified collagen type VI from human placenta.

Application Details

Application Notes: ELISA: 1/200 (OD ≥ 500). RIA. Immunofluorescence: 1/80. Immunohistology on Frozen and Paraffin Embedded Sections. For paraffin: 1/400-1/1000, 60 min at RT or 2-8 °C over night for human collagen type VI, lower dilution is recommended for other species). Positive Control: Human placenta. Pretreatment : After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS containing no BSA or preservative.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for COL6

Target: COL6 (Collagen, Type VI (COL6))

Alternative Name: collagen type vi

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea.

UniProt: P12109