CEACAM7 antibody

Catalog No. ABIN5541193

Cited in 6+ publications. The Mouse Monoclonal anti-CEACAM7 antibody (Clone BAC2) (ABIN5541193) specifically detects CEACAM7 in ICC, IF, FACS, IHC (fro) and EIA. The antibody is reactive with Human samples.

Quick Overview for CEACAM7 antibody (ABIN5541193)

Target: CEACAM7 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 7 (CEACAM7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM7 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: BAC2

Product Details anti-CEACAM7 Antibody

Specificity: This antibody recognizes CEACAM7.

Purification: Affinity Chromatography on Protein G

Immunogen: CEACAM7/Fc fusion protein

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200. Flow cytometry: 1.2 μg/10 6 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2 without preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-CEACAM7 antibody (ABIN5541193)

Yue, Goldstein, Hollingsworth, Kaul, Brand, Haab: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, (2009) (PubMed).

Bratke, Goettsching, Kuepper, Geyer, Luttmann, Virchow: "Interleukin-4 suppresses the cytotoxic potential of in vitro generated, adaptive regulatory CD4 T cells by down-regulation of granzyme B." in: Immunology, Vol. 127, Issue 3, pp. 338-44, (2009) (PubMed).

Thomas, Zhu, Schnaar, Alves, Konstantopoulos: "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15647-55, (2008) (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

Fahlgren, Baranov, Frängsmyr, Zoubir, Hammarström, Hammarström: "Interferon-gamma tempers the expression of carcinoembryonic antigen family molecules in human colon cells: a possible role in innate mucosal defence." in: Scandinavian journal of immunology, Vol. 58, Issue 6, pp. 628-41, (2003) (PubMed).

Schölzel, Zimmermann, Schwarzkopf, Grunert, Rogaczewski, Thompson: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, (2000) (PubMed).

Target Details for CEACAM7

Target: CEACAM7 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 7 (CEACAM7))

Alternative Name: ceacam7

Background: CEA-related cell adhesion molecule 7 (CEACAM7, CGM2) belongs to the carcinoembryonic antigen (CEA) family (1). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein which is only found on the apical surface of highly differentiated epithelial cells adult colon and on a small epithelial cell population in pancreatic ducts (2). CEACAM7 expression is completely lost upon malignant transformation already in hyperplastic polyps and early adenomas as well as in adenocarcinomas of the colon (2). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant-like A domain.

UniProt: Q14002