DIABLO antibody (AA 56-239)

Catalog No. ABIN5541224

This Mouse Monoclonal antibody specifically detects DIABLO in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for DIABLO antibody (AA 56-239) (ABIN5541224)

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT19F2

Product Details anti-DIABLO Antibody

Binding Specificity: AA 56-239

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human SMAC/Diablo (56-239aa) purified from E. coli

Isotype: IgG2a

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.Recommended starting dilution is 1:3000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: diablo,smac

Background: SMAC/Diablo is one of the proapoptotic proteins. SMAC promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. In healthy cells, SMAC is a mitochondrial protein, but when cells undergo apoptosis, SMAC is released into the cytosol. SMAC is ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes

UniProt: Q9NR28

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity