DAB1 antibody

Catalog No. ABIN5541230

This Rabbit Polyclonal antibody specifically detects DAB1 in WB, IF, IHC (p), ICC and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for DAB1 antibody (ABIN5541230)

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA)

Product Details anti-DAB1 Antibody

Specificity: The antibody detects endogenous levels of total Dab1 protein.

Purification: Immunoaffinity Chromatogrphy

Immunogen: Synthetic peptide from human Dab1 (internal epitope)

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 20 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Immunocytochemistry/Immunofluorescence: 1/100 - 1/500. Western blot: 1/500 - 1/1000. ELISA: 1/20000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 50 % glycerol Preservatives: 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Alternative Name: dab1

Background: < span class=RECOMMENDED> < span class=FULL> Disabled homolog 1 is an adapter molecule in neurogenesis.

UniProt: O75553