EGFL7 antibody (C-Term)

Catalog No. ABIN5541241

The Rabbit Polyclonal anti-EGFL7 antibody (ABIN5541241) specifically detects EGFL7 in WB, IP, ICC and IF. The antibody is reactive with Mouse samples.

Quick Overview for EGFL7 antibody (C-Term) (ABIN5541241)

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFL7 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-EGFL7 Antibody

Binding Specificity: AA 181-275, C-Term

Specificity: This antibody recognizes VE-Statin/EGFL7.

Purification: Protein A chromatography

Immunogen: Highly pure recombinant mouse EGFL7 (Glu181-Leu275 C-terminus) derived from E. coli

Isotype: IgG

Application Details

Application Notes: Western blot: 1-5 μg/mL. Immnunocytochemistry. Immunoprecipitation.

Restrictions: For Research Use only

Handling

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for EGFL7

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Alternative Name: egfl7

Background: EGFL7 (EGF-like domain-containing protein 7) regulates vascular tubulogenesis in vivo. During sprouting angiogenesis, groups of endothelial cells (ECs) migrate together in units called sprouts. EGFL7 regulates the proper spatial organization of ECs within each sprout and influences their collective movement. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro. EGFL7 is an ~ 30 kDa secreted protein that contain an Emilin-like (EMI) domain (a multimerization motif), and 2 epidermal growth factor (EGF) domains, one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble like extracellular matrix (ECM) proteins and, thus, could incorporate into ECM. EGFL7 has been reported to stimulate cell adhesion as well as motility in a manner similar to ECM proteins. EGFL7 has been shown to be primarily expressed by developing ECs but also by primordial germ cells and some central nervous system neurons. Interestingly, EGFL7 expression markedly decreases in ECs in postnatal life, but can be strongly up-regulated after various tissue injuries that lead to increased angiogenic responses.

UniProt: Q9QXT5