EGFL7 antibody (C-Term)

Catalog No. ABIN5541243

The Rabbit Polyclonal anti-EGFL7 antibody is suitable to detect EGFL7 in samples from Human. It has been validated for WB, IP, ICC, IF and IHC (fro).

Quick Overview for EGFL7 antibody (C-Term) (ABIN5541243)

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFL7 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-EGFL7 Antibody

Binding Specificity: AA 178-273, C-Term

Specificity: This antibody recognizes VE-Statin / EGFL7 at C-term.

Purification: Protein A chromatography

Immunogen: Highly pure recombinant human EGFL7 (Pro178-Ser273) C-terminus derived from insect cells

Isotype: IgG

Application Details

Application Notes: Western blot: 2-5 μg/mL. Immunohistochemistry on Frozen sections. Immnunocytochemistry. Immunoprecipitation.

Restrictions: For Research Use only

Handling

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for EGFL7

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Alternative Name: egfl7

Background: EGFL7 (EGF-like domain-containing protein 7) regulates vascular tubulogenesis in vivo. During sprouting angiogenesis, groups of endothelial cells (ECs) migrate together in units called sprouts. EGFL7 regulates the proper spatial organization of ECs within each sprout and influences their collective movement. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro. EGFL7 (VE-Statin) is an ~ 30 kDa secreted protein that contains an Emilin-like (EMI) domain (a multimerization motif), and two epidermal growth factor (EGF) domains, one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble like extracellular matrix (ECM) proteins and, thus, could incorporate into ECM. EGFL7 has been reported to stimulate cell adhesion as well as motility in a manner similar to ECM proteins. EGFL7 has been shown to be primarily expressed by developing ECs but also by primordial germ cells and some central nervous system neurons. Interestingly, EGFL7 expression markedly decreases in ECs in postnatal life, but can be strongly up-regulated after various tissue injuries that lead to increased angiogenic responses.

UniProt: Q9UHF1