GAB2 antibody (Tyr643)

Catalog No. ABIN5541265

The Rabbit Polyclonal anti-GAB2 antibody (ABIN5541265) specifically detects GAB2 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GAB2 antibody (Tyr643) (ABIN5541265)

Target: GAB2 (GRB2-Associated Binding Protein 2 (GAB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAB2 Antibody

Binding Specificity: Tyr643

Specificity: The antibody detects endogenous levels of Gab2 only when phosphorylated at tyrosine 643.

Purification: Affinity chromatography using epitope-specific peptide

Immunogen: Peptide sequence around phosphorylation site of tyrosine 643(V-D-Y(p)-V-Q) derived from Human Gab2 (KLH-conjugated)

Application Details

Application Notes: Western blot: 1:500~1:1000. Immunohistochemistry on paraffin sections: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAB2

Target: GAB2 (GRB2-Associated Binding Protein 2 (GAB2))

Alternative Name: gab2

Background: This gene is a member of the GRB2-associated binding protein (GAB) gene family and is similar to the GAB1 gene. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.

UniProt: Q9UQC2

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, BCR Signaling, Warburg Effect