TIMD4 antibody (AA 1-288)

Catalog No. ABIN5541407

This Rat Monoclonal antibody specifically detects TIMD4 in FACS, IF, IHC (p) and ICC. It exhibits reactivity toward Mouse.

Quick Overview for TIMD4 antibody (AA 1-288) (ABIN5541407)

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TIMD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: RMT4-54

Product Details anti-TIMD4 Antibody

Binding Specificity: AA 1-288

Specificity: Clone RMT4-54 specifically recognises mouse Tim-4. Studies have shown that clone RMT4-54 significantly inhibits the efficient phagocytosis of apoptotic cells by PRMs (peritoneal resident Mac1 + cells), but not PEMs (peritoneal exudate Mac1 cells) [Nakayama, 2009]. Species: Mouse.

Purification: Affinity chromatography on Protein G

Immunogen: Mouse TIMD4 (amino acids 1-288), coupled with the Fc portion of mouse IgG2a

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: 1/25-1/200, use 10 μL of this suggested working dilution to label 10e6 cells in 100 μL. Immunofluorescence. Immunohistochemistry on Paraffin sections. Positive control: Mouse spleen. For functional assays Cat.-No. AM60019LE-N is recommended.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TIMD4

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Alternative Name: timd4

Background: TIMD4 / TIM-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, exclusively expressed by antigen-presenting cells, which acts as the natural ligand of TIM-1, involved in T-cell expansion and cytokine production. TIMD4 is reported to be crucial for the phagocytosis of apoptotic cells by peritoneal macrophages, and studies have shown that interaction between TIM-1 on Th2 cells and TIM-4 on DCs, enhances Th2 cell function. The TIMD4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma.

UniProt: Q6U7R4

Pathways: Cancer Immune Checkpoints