MESP1 antibody (AA 1-268)

Catalog No. ABIN5541523

The Rabbit Polyclonal anti-MESP1 antibody (ABIN5541523) specifically detects MESP1 in WB, ICC, IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for MESP1 antibody (AA 1-268) (ABIN5541523)

Target: MESP1 (Mesoderm Posterior 1 (MESP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MESP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MESP1 Antibody

Binding Specificity: AA 1-268

Specificity: This antibody detetcs Human MesP1. Other species not tested.

Purification: Protein A Chromatography

Immunogen: Highly pure (> 95%) recombinant Human MesP1 (Met1-Lys268) from produced in insect cells (Cat.-No AR31057PU-N).

Application Details

Application Notes: Western blot (1-5 μg/mL). Immunofluorescence. Immunohistochemistry on Frozen Sections (1/200).

Restrictions: For Research Use only

Handling

Buffer: 5 mM PBS, pH 7.2 without preservatives or stabilizers

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MESP1

Target: MESP1 (Mesoderm Posterior 1 (MESP1))

Alternative Name: mesp1

Background: ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1 (1,2). As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis (2,3). It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation (4). MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1.

Pathways: Regulation of Muscle Cell Differentiation