BUB3 antibody (AA 1-328)

Catalog No. ABIN5541713

This Mouse Monoclonal antibody specifically detects BUB3 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for BUB3 antibody (AA 1-328) (ABIN5541713)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT2H6

Product Details anti-BUB3 Antibody

Binding Specificity: AA 1-328

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human BUB3 (1-328aa) purified from E. coli.

Isotype: IgG2b

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: bub3

Background: BUB3 (budding uninhibited by benzimidazoles 3 homolog) is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.

UniProt: O43684

Pathways: Maintenance of Protein Location