P-Selectin antibody

Catalog No. ABIN5541731

This Mouse Monoclonal antibody specifically detects P-Selectin in WB, FACS, IP, ICC, IF and Func. It exhibits reactivity toward Human and Primate.

Quick Overview for P-Selectin antibody (ABIN5541731)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Functional Studies (Func)

Clone: AK4

Product Details anti-P-Selectin Antibody

Specificity: This antibody recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes it is relocated to the plasma membrane upon activation.

Purification: Protein-A affinity chromatography

Immunogen: Human platelets

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.5 μg/mL. Immunoprecipitation. Western blot. Immunocytochemistry. Functional application: Blocking.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline (PBS) Preservatives: 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: cd62p,p-selectin

Background: CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.

UniProt: P16109