CEACAM6 antibody

Catalog No. ABIN5541783

Product Details anti-CEACAM6 Antibody

Target: CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: 9A6 recognizes exclusively CEACAM6 transiently expressed on the cell surface of transfected BOSC cells (Fig. 1). 9A6 can be used to distinguish CEACAM6 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules, namely CEACAM1 (BGP/CD66a), CEACAM3 (CGM1/CD66d), CEACAM4 (CGM7), CEACAM6 (NCA/CD66c), CEACAM7 (CGM2), CEACAM8 (CGM6/CD66b) and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members (3 and Fig.1). 9A6 was included and characterized in the framework of the VIth Leucocyte Typing Workshop. Species: Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Tumor cell lines expressing CEACAM6 (1,2)

Clone: 9A6

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. Western blot: 4 μg/mL. Flow Cytometry: 1.2 μg/10 6 cells. Immunohistology on Cryo-Sections: 1-2 μg/10 For various aplications see list of References.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-CEACAM6 antibody (ABIN5541783)

Kanderová, Hrusák, Kalina: "Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells." in: Experimental hematology, Vol. 38, Issue 8, pp. 653-660.e1, (2010) (PubMed).

Singer, Scheffrahn, Kammerer, Suttorp, Ergun, Slevogt: "Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells." in: PLoS ONE, Vol. 5, Issue 1, pp. e8747, (2010) (PubMed).

Carvalho, Barnich, Sivignon, Darcha, Chan, Stanners, Darfeuille-Michaud: "Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM." in: The Journal of experimental medicine, Vol. 206, Issue 10, pp. 2179-89, (2009) (PubMed).

Sémiramoth, Gleizes, Turbica, Sandré, Gorges, Kansau, Servin, Chollet-Martin: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, (2009) (PubMed).

Yue, Goldstein, Hollingsworth, Kaul, Brand, Haab: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, (2009) (PubMed).

Thomas, Zhu, Schnaar, Alves, Konstantopoulos: "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15647-55, (2008) (PubMed).

Chan, Camacho-Leal, Stanners: "Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice." in: PLoS ONE, Vol. 2, Issue 12, pp. e1353, (2007) (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

Kalina, Vaskova, Mejstrikova, Madzo, Trka, Stary, Hrusak: "Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens." in: BMC cancer, Vol. 5, pp. 38, (2005) (PubMed).

Schmitter, Agerer, Peterson, Munzner, Hauck: "Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens." in: The Journal of experimental medicine, Vol. 199, Issue 1, pp. 35-46, (2004) (PubMed).

Marinkovich, Lunstrum, Burgeson: "The anchoring filament protein kalinin is synthesized and secreted as a high molecular weight precursor." in: The Journal of biological chemistry, Vol. 267, Issue 25, pp. 17900-6, (1992) (PubMed).

Target Details for CEACAM6

Target: CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6))

Alternative Name: cd66c,ceacam6 (CEACAM6 Products)

Synonyms: CD66c antibody, CEAL antibody, NCA antibody, carcinoembryonic antigen-related cell adhesion molecule 6 antibody, carcinoembryonic antigen related cell adhesion molecule 6 antibody, Ceacam6 antibody, CEACAM6 antibody

Background: CEA-related cell adhesion molecule 6 (CEACAM6, NCA) belongs to the carcinoembryonic antigen (CEA) gene family (13). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 90,000 which is strongly expressed on epithelial cells of the fetal and adult gastrointestinal tract, epithelia of glandular tissues, squamous epithelial cell of the tongue, esophagus and cervix as well as on granulocytes (Fig. 2 Ref. 4, 10). CEACAM6 expression is upregulated in many adenocarcinomas (4) and leukemias (5). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant-like A and B domain.

UniProt: P40199