GFRA4 antibody

Catalog No. ABIN5541834

The Chicken Polyclonal anti-GFRA4 antibody is suitable to detect GFRA4 in samples from Human. It has been validated for WB, IF, ICC and EIA.

Quick Overview for GFRA4 antibody (ABIN5541834)

Target: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFRA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA)

Product Details anti-GFRA4 Antibody

Specificity: This antibody detects Human GFRa-4 and doesn't cross-react with hGFRa-1, hGFRa-2, hGFRa-3. Other species not tested.

Purification: Affinity Chromatography

Immunogen: Recombinant His-tagged human GFRa-4 protein produced using CHO-based. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

Isotype: IgY

Application Details

Application Notes: ELISA: 1/160000-1/320000. Western blot: 1/5000-1/10000. Immunofluorescence: 1/1000-1/3000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Concentrated Ammonium Sulphate in PBS pH 7.4

Storage: 4 °C

Storage Comment: Upon receipt, store at 2-8°C. As product is (NH4) 2 SO 4 precipitate, mix well by pipetting or vortexing prior use. Shelf life: One year from despatch.

Target Details for GFRA4

Target: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Alternative Name: gfra4,gdnfr-alpha 4

Background: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).

UniProt: Q9GZZ7