GZMK antibody

Catalog No. ABIN5541917

Product Details anti-GZMK Antibody

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMK antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Granzyme K (GZMK). Species: Human.

Purification: Affinity Chromatography on Protein G

Immunogen: GM24C3 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the Granzyme K cDNA

Clone: GM24C3

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-GZMK antibody (ABIN5541917)

Krause, Behrends, Borowski, Lohrmann, Lang, Myrtek, Lorenzen, Virchow, Luttmann, Friedrich: "Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor alpha1." in: Molecular immunology, Vol. 43, Issue 11, pp. 1799-807, (2006) (PubMed).

Bratke, Kuepper, Bade, Virchow, Luttmann: "Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood." in: European journal of immunology, Vol. 35, Issue 9, pp. 2608-16, (2005) (PubMed).

Target Details for GZMK

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Alternative Name: granzyme k (gzmk) (GZMK Products)

Synonyms: TRYP2 antibody, granzyme K antibody, GZMK antibody, Gzmk antibody

Background: Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.

UniProt: P49863