GZMK antibody

Catalog No. ABIN5541918

The Mouse Monoclonal anti-GZMK antibody is suitable to detect GZMK in samples from Human. It has been validated for FACS, EIA and ELISA (Capture).

Quick Overview for GZMK antibody (ABIN5541918)

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMK antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: GM26E7

Product Details anti-GZMK Antibody

Specificity: This antibody reacts to Granzyme K (GZMK). Species: Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Genetic immunisation with cDNA encoding human Granzyme K

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. ELISA (capture): Clone GM-6C3 as detection antibody. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GZMK

Target: GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))

Alternative Name: granzyme k (gzmk)

Background: Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.

UniProt: P49863