K-RAS antibody (AA 1-186)

Catalog No. ABIN5541964

This anti-K-RAS antibody is a Mouse Monoclonal antibody detecting K-RAS in WB, IF, FACS, ICC and EIA. Suitable for Human.

Quick Overview for K-RAS antibody (AA 1-186) (ABIN5541964)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA)

Clone: AT2F8

Product Details anti-K-RAS Antibody

Binding Specificity: AA 1-186

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human KRAS (1-186aa) purified from E. coli.

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. In Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: kras

Target Type: Viral Protein

Background: KRAS acts as a molecular on/off switch. Once it is turned on, it recruits and activates proteins necessary for the propagation of growth factor and other receptors' sigl such as c-Raf and PI 3-kise. KRAS binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the termil phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, KRAS is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase-activating protein (GAP) class, for example RasGAP. In turn KRAS can bind to proteins of the Guanine Nucleotide Exchange Factor (GEF) class, for example SOS1, which forces the release of bound nucleotide (GDP). Subsequently, KRAS binds GTP present in the cytosol and the GEF is released from ras-GTP.

UniProt: P01116