Topoisomerase II alpha antibody (C-Term)

Catalog No. ABIN5542013

This Mouse Monoclonal antibody specifically detects Topoisomerase II alpha in WB, IF, FACS, IHC (p), IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Topoisomerase II alpha antibody (C-Term) (ABIN5542013)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 1C5

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: C-Term

Specificity: This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.

Cross-Reactivity (Details): Does not rect with Mouse (WR19L, NIH/3T3), Rat (PC12, Rat-1) or Hamster (BHK) on Western blot.

Purification: Protein A agarose

Immunogen: Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa

Isotype: IgG2a

Application Details

Application Notes: Western blot: 0.1-0.5 μg/mL for chemiluminescence detection system. Immunoprecipitation: 1-10 μg/200-300 μg/mL of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary Microwave oven 2 times for 10 minutes aech in 10 mM citrate buffer ( pH 6.5). Immunocytochemistry: 1-10 μg/mL. Flow cytometry: 0.1-1 μg/mL (final contentration).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: top2a

Background: < div dir=ltr data-font-name=g_font_p0_4 data-canvas-width=519.3466821444035> Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well < span style=font-size: 12px> as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases