Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Topoisomerase II alpha antibody (C-Term)

This Mouse Monoclonal antibody specifically detects Topoisomerase II alpha in WB, IF, FACS, IHC (p), IP and ICC. It exhibits reactivity toward Human.
Catalog No. ABIN5542013

Quick Overview for Topoisomerase II alpha antibody (C-Term) (ABIN5542013)

Target

See all Topoisomerase II alpha (TOP2A) Antibodies
Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity

  • 142
  • 63
  • 32
  • 5
  • 5
  • 3
  • 3
  • 3
  • 1
Human

Host

  • 104
  • 39
Mouse

Clonality

  • 91
  • 52
Monoclonal

Conjugate

  • 78
  • 9
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Topoisomerase II alpha antibody is un-conjugated

Application

  • 100
  • 61
  • 52
  • 46
  • 22
  • 18
  • 13
  • 7
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone

1C5
  • Binding Specificity

    • 19
    • 14
    • 10
    • 9
    • 8
    • 7
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.

    Cross-Reactivity (Details)

    Does not rect with Mouse (WR19L, NIH/3T3), Rat (PC12, Rat-1) or Hamster (BHK) on Western blot.

    Purification

    Protein A agarose

    Immunogen

    Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa

    Isotype

    IgG2a
  • Application Notes

    Western blot: 0.1-0.5 μg/mL for chemiluminescence detection system. Immunoprecipitation: 1-10 μg/200-300 μg/mL of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary Microwave oven 2 times for 10 minutes aech in 10 mM citrate buffer ( pH 6.5). Immunocytochemistry: 1-10 μg/mL. Flow cytometry: 0.1-1 μg/mL (final contentration).

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

    Preservative

    Azide free

    Storage

    -20 °C

    Storage Comment

    Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
  • Target

    Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

    Alternative Name

    top2a

    Background

    < div dir=ltr data-font-name=g_font_p0_4 data-canvas-width=519.3466821444035> Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well < span style=font-size: 12px> as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.

    UniProt

    P11388

    Pathways

    Cell Division Cycle, Mitotic G1-G1/S Phases
You are here:
Chat with us!