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PDPK1 antibody (AA 457-556)

Name: PDPK1 antibody (AA 457-556)
Brand: antibodies-online
SKU: ABIN5542259
Price: 533.5 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects PDPK1 in WB, ELISA, IHC, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (PDPK1 antibody (AA 457-556))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PDPK1 antibody (AA 457-556))

ELISA

Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate. (PDPK1 antibody (AA 457-556))

Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate. (PDPK1 antibody (AA 457-556))

Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red). (PDPK1 antibody (AA 457-556))

FACS

Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). (PDPK1 antibody (AA 457-556))

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PDPK1 mouse mAb with DAB staining. (PDPK1 antibody (AA 457-556))

IHC

7 images

Catalog No. ABIN5542259

Quick Overview for PDPK1 antibody (AA 457-556) (ABIN5542259)

Target

See all PDPK1 Antibodies

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity

Human, Mouse, Rat, Monkey

Host

Mouse

Clonality

Monoclonal

Conjugate

This PDPK1 antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone

7E4G11

Discover our top product PDPK1 Primary Antibody

Binding Specificity
24

16

15

15

15

15

8

7

6

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 457-556

Purpose

PDPK1 Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.

Isotype

IgG1
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody
PDPK1 Reactivity: Human, Mouse, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 150-429) antibody
PDPK1 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-150) antibody
PDPK1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-429) antibody
PDPK1 Reactivity: Human WB, PLA Host: Mouse Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody
PDPK1 Reactivity: Human, Rat, Monkey WB, ELISA, IHC, ICC, FACS Host: Mouse Monoclonal 4A11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 524-556), (C-Term) antibody
PDPK1 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 83-338) antibody
PDPK1 Reactivity: Human WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Internal Region) antibody
PDPK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2E2 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 277-556) antibody
PDPK1 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50 - 1/200

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name

PDPK1

Background

Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1.

Molecular Weight

63.2 kDa

UniProt

O15530

Pathways

PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals