RAD50 antibody (AA 228-359)

Catalog No. ABIN5542671

The Mouse Monoclonal anti-RAD50 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect RAD50 in samples from Human and Rat.

Quick Overview for RAD50 antibody (AA 228-359) (ABIN5542671)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 3D2G10

Product Details anti-RAD50 Antibody

Binding Specificity: AA 228-359

Purpose: RAD50 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human RAD50 (AA: 228-359) expressed in E. Coli.

Isotype: IgG2a

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Alternative Name: RAD50

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Molecular Weight: 154 kDa

UniProt: Q92878

Pathways: DNA Damage Repair, Protein targeting to Nucleus