Arrestin 3 antibody (Internal Region)

Catalog No. ABIN374557

This Goat Polyclonal antibody specifically detects Arrestin 3 in WB, IP, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for Arrestin 3 antibody (Internal Region) (ABIN374557)

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Arrestin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Arrestin 3 Antibody

Binding Specificity: Internal Region

Sequence: C-HDHIPLPRPQ S

Specificity: This antibody is expected to recognizse both reported isoforms NP_004304.1 and NP_945355.1. No cross-reactivity is expected with Arrestin beta 1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004304.1, NP_945355.1. Genename: ARRB2

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Arrestin 3 antibody (ABIN374557)

Cassidy, Slyne, OKelly, Traynor, Conlon, Johnston, Slattery, Ryan, McMorrow: "Urinary biomarkers of chronic allograft nephropathy." in: Proteomics. Clinical applications, Vol. 9, Issue 5-6, pp. 574-85, (2015) (PubMed).

Montserrat, Ramírez-Bajo, Xia, Sancho-Martinez, Moya-Rull, Miquel-Serra, Yang, Nivet, Cortina, González, Izpisua Belmonte, Campistol: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).

Zakrzewicz, Krasteva, Wilhelm, Dietrich, Wilker, Padberg, Wygrecka, Grau: "Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys." in: Immunobiology, Vol. 216, Issue 7, pp. 854-61, (2011) (PubMed).

Target Details for Arrestin 3

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Alternative Name: Arrestin beta-2 / ARRB2

Background: Members of arrestin/beta arrestin protein family are thought to participate in agonist mediated desensitization of G protein coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors.Synonyms: ARB2, ARR2, Beta-arrestin-2

Molecular Weight: 46.1 kDa according to NP_004304.1 and 44.4 according to NP_945355.1.

Gene ID: 409, 9606

UniProt: P32121

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Phototransduction, Thromboxane A2 Receptor Signaling