Amphiphysin antibody (Internal Region)

Catalog No. ABIN374682

The Goat Polyclonal anti-Amphiphysin antibody (ABIN374682) specifically detects Amphiphysin in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for Amphiphysin antibody (Internal Region) (ABIN374682)

Target: Amphiphysin (AMPH)

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Amphiphysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Amphiphysin Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to Amphiphysin.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.1 - 0.5 μg/mL. Immunohistochemistry on paraffin sections: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Amphiphysin

Target: Amphiphysin (AMPH)

Alternative Name: Amphiphysin

Background: Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.Synonyms: AMPH, AMPH1

Gene ID: 273, 9606

UniProt: P49418