beta Catenin antibody (pSer33, pSer37) (AbBy Fluor® 750)

Catalog No. ABIN5557424

This anti-beta Catenin antibody is a Rabbit Polyclonal antibody detecting beta Catenin in WB, FACS, IF (cc) and IF (p). Suitable for Human, Mouse and Rat.

Quick Overview for beta Catenin antibody (pSer33, pSer37) (AbBy Fluor® 750) (ABIN5557424)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Catenin antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-beta Catenin Antibody (AbBy Fluor® 750)

Binding Specificity: pSer33, pSer37

Specificity: These phosphorylation sites are homologous to Ser33 + Ser37 in Mouse and Rat.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugatedsynthetic phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Ser33/37

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: beta Catenin

Background:

Synonyms: CTNNB, MRD19, armadillo, Catenin beta-1, Beta-catenin, CTNNB1, OK/SW-cl.35, PRO2286

Background: Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML.

Gene ID: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism