Recombinant OCT4 antibody

Catalog No. ABIN5557502

The Rabbit Monoclonal anti-OCT4 antibody (ABIN5557502) specifically detects OCT4 in WB, IF, ICC, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Recombinant OCT4 antibody (ABIN5557502)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-OCT4 Antibody

Purpose: Oct4 Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant human Oct 4 protein, around 1-100aa.

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1 μg/Test), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct 4

Background:

Synonyms: MGC22487, Oct 3, Oct 4, Oct-3, Oct-4, OCT3, Oct4, Octamer binding protein 3, Octamer-binding protein 3, Octamer binding protein 4, Octamer binding transcription factor 3, Octamer-binding transcription factor 3, Octamer-4, POU domain transcription factor OCT4, OTF3, OTF4, OTF 3, OTF 4, OTF-3, POU class 5 homeobox 1, POU domain, class 5, transcription factor 1, POU domain class 5 transcription factor 1, PO5F1_HUMAN, POU type homeodomain containing DNA binding protein , POU5F1.

Background: Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Gene ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance