SMAD3 antibody (pSer423, pSer425)

Catalog No. ABIN5557525

The Rabbit Monoclonal anti-SMAD3 antibody has been validated for WB, IHC (p) and IF (cc). It is suitable to detect SMAD3 in samples from Human, Mouse and Rat.

Quick Overview for SMAD3 antibody (pSer423, pSer425) (ABIN5557525)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 5C5

Product Details anti-SMAD3 Antibody

Binding Specificity: pSer423, pSer425

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SMAD3 around the phosphorylation site of Ser423/425:CS(p-S)V(p-S)

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: Smad3

Background:

Synonyms: Smad3 phospho Ser423, Smad3 phospho S423, p-Smad3 Ser423, hMAD 3, hSMAD3, HSPC193, JV15 2, JV152, MAD mothers against decapentaplegic Drosophila homolog 3, MAD3, MADH 3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD 3, SMAD, SMAD-3, SMAD3_HUMAN.

Background: Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8), the common partner, (Smad4) that functions via its interaction to the various Smads, and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

Gene ID: 4088

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy