CRISPR-Cas9 antibody

Catalog No. ABIN5557542

This Mouse Monoclonal antibody specifically detects in WB. It exhibits reactivity toward Human.

Quick Overview for CRISPR-Cas9 antibody (ABIN5557542)

Target: CRISPR-Cas9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Clone: 1E8

Product Details

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (100-200aa)

Isotype: IgM

Application Details

Application Notes: WB 1:500-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: CRISPR-Cas9

Background:

Synonyms: CRISPR, Cas9, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, SpCas9, csn1

Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Gene ID: 901176

UniProt: Q99ZW2