N6-Methyladenosine antibody

Catalog No. ABIN5563963

Cited in 1 publication. The Mouse Monoclonal anti-N6-Methyladenosine antibody (Clone 17-3-4-1) (ABIN5563963) specifically detects N6-Methyladenosine in DB and IP. The antibody is reactive with Human and Saccharomyces cerevisiae samples.

Quick Overview for N6-Methyladenosine antibody (ABIN5563963)

Target: N6-Methyladenosine

Reactivity: Human, Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Immunoprecipitation (IP)

Clone: 17-3-4-1

Product Details

Purpose: N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)

Specificity: Budding Yeast, Human, Other (Wide Range)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against 6-Methyladenosine conjugated to BSA.

Isotype: IgG1 kappa

Application Details

Application Notes: For N6-Methyladenosine (m6A), we also offer an AbFlex N6-Methyladenosine (m6A) Recombinant Antibody (rAb). For details, see Catalog No. 91261.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References

Bodi, Button, Grierson, Fray: "Yeast targets for mRNA methylation." in: Nucleic acids research, Vol. 38, Issue 16, pp. 5327-35, (2010) (PubMed).

Target Details

Target: N6-Methyladenosine

Alternative Name: N6-Methyladenosine (m6A)

Target Type: Chemical

Background: N6-Methyladenosine (m6A) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3' UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.