SNCA antibody (Atto 594)

Catalog No. ABIN5564075

This Mouse Monoclonal antibody specifically detects SNCA in WB, ELISA, IF, ICC and DB. It exhibits reactivity toward Mouse.

Quick Overview for SNCA antibody (Atto 594) (ABIN5564075)

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNCA antibody is conjugated to Atto 594

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 10H7

Product Details anti-SNCA Antibody (Atto 594)

Specificity: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.,Binds within the 101-130 aa of human ASYN, determined using DB and ELISA with corresponding peptides. Detects ~14 kDa band corr to ASYN. ~40 kDa is a trimer. Does not detect truncated ASYN (aa 1-114)

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Mouse alpha synuclein aggregate

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• DB (1:1000)
• ICC/IF (1:100)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5564075 was sufficient for detection of Alpha Synuclein in 15 μg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: Alpha Synuclein

Background: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

Gene ID: 20617

NCBI Accession: NP_001035916

UniProt: O55042

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity