Caspase 2 antibody (Subunit p19)

Catalog No. ABIN5564277

The Rat Monoclonal anti-Caspase 2 antibody is suitable to detect Caspase 2 in samples from Human, Mouse, Rat, Dog and Monkey. It has been validated for ELISA, IHC, ICC and FACS.

Quick Overview for Caspase 2 antibody (Subunit p19) (ABIN5564277)

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Reactivity: Human, Mouse, Rat, Dog, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This Caspase 2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 10C6

Product Details anti-Caspase 2 Antibody

Binding Specificity: Subunit p19

Purpose: anti-Caspase-2, mAb (10C6)

Specificity: Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2.

Characteristics: Monoclonal Antibody. Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Isotype: Rat IgG2akappa. Clone: 10C6. Applications: ELISA, FACS, ICC, IHC. Liquid. In PBS containing 0.02 % sodium azide. Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant human caspase-2 (p19 subunit).

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Alternative Name: Caspase-2

Background:

Alternate Names/Synonyms: CASP-2, Protease ICH-1, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2, NEDD-2

Product Description: Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.

UniProt: P42575

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway