RASGRP2 antibody (C-Term)

Catalog No. ABIN5564284

The Rabbit Polyclonal anti-RASGRP2 antibody (ABIN5564284) specifically detects RASGRP2 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for RASGRP2 antibody (C-Term) (ABIN5564284)

Target: RASGRP2 (RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RASGRP2 Antibody

Binding Specificity: C-Term

Purpose: anti-RasGRP2, pAb (IG-09)

Specificity: Recognizes human and mouse RasGRP2.

Characteristics: Polyclonal Antibody. Recognizes human and mouse RasGRP2. Source: Rabbit. Applications: WB. Liquid. In PBS containing 2 mg/mL BSA and 0.05 % sodium azide. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.

Purification: Antigen affinity purified.

Purity: Antigen affinity purified.

Immunogen: Synthetic peptide corresponding to a human RasGRP2 sequence located at the C-terminus of the RasGEF domain.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS containing 2 mg/mL BSA and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.Keep sterile.

Storage: 4 °C

Storage Comment:

+4°C

+4°C

Stable for at least 6 months after receipt when stored at +4°C.

Expiry Date: 6 months

Target Details for RASGRP2

Target: RASGRP2 (RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2))

Alternative Name: RasGRP2

Background:

Alternate Names/Synonyms: CalDAG-GEFI, Ras Guanyl Nucleotide-releasing Protein 2, Ras Guanyl Releasing Protein 2, CDC25-like Protein, CDC25L, Calcium and DAG-regulated Guanine Nucleotide Exchange Factor I, MCG7

Product Description: RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.

UniProt: Q7LDG7

Pathways: TCR Signaling