AKT 1/2/3 antibody (Biotin)

Catalog No. ABIN5596660

Supplier Product No.: 200-406-n98

The Biotin-conjugated Rabbit Polyclonal anti-AKT 1/2/3 antibody (ABIN5596660) specifically detects AKT 1/2/3 in WB, IHC, FACS, ELISA, IP and FM. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for AKT 1/2/3 antibody (Biotin) (ABIN5596660)

Target: AKT 1/2/3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-AKT 1/2/3 Antibody (Biotin)

Supplier Product No.: 200-406-n98

Supplier: Rockland

Purpose: AKT Antibody Biotin Conjugated

Cross-Reactivity (Details): AKT Antibody reacts with the AKT from human tissues.

Characteristics: Synonyms: rabbit anti-AKT Antibody Biotin Conjugated, Biotin conjugated rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1 Antibody, AKT2 Antibody, AKT3 Antibody, Biotin, BAC

Purification: Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography.

Immunogen:

Immunogen: Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Biotin Conjugated AKT Antibody is tested for western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:500 - 1:2,000

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1, AKT2, AKT3

Background: Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Gene ID: 10000

NCBI Accession: NP_001014431

UniProt: P31749