TBC1D4 antibody (pThr642)

Catalog No. ABIN5596682

Supplier Product No.: 600-401-j32

This Rabbit Polyclonal antibody specifically detects TBC1D4 in ELISA, WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TBC1D4 antibody (pThr642) (ABIN5596682)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TBC1D4 Antibody

Binding Specificity: pThr642

Supplier Product No.: 600-401-j32

Supplier: Rockland

Purpose: TBC1D4 phospho T642 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-TBC1D4 pT642 antibody, phosphorylated TBC1D4 antibody, TBC1 domain family member 4, Akt substrate of 160 kDa, AS160, TBC1D4, KIAA0603

Purification: Anti-TBC1D4 [p Thr642] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: TBC1D4 [p Thr642] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the pT649 site of human TBC1D4 [p Thr642].

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-TBC1D4 pT642 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~160 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: Tbc1d4

Background: Background: TBC1D4 [p Thr642] is associated with glucose uptake by promoting insulin-induced glucose transporter. The Akt substrate 160 pT649 activation by Akt has been associated with the GLUT4 translocation upon insulin activation in 3T3-L1 cells. Anti-TBC1D4 [p Thr642] antibody is ideal for investigators interested in Signal Transduction research.

UniProt: Q8BYJ6