Calreticulin antibody (C-Term)

Catalog No. ABIN5596697

Supplier Product No.: 600-401-j41

This Rabbit Polyclonal antibody specifically detects Calreticulin in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Calreticulin antibody (C-Term) (ABIN5596697)

Target: Calreticulin (CALR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Calreticulin Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-j41

Supplier: Rockland

Purpose: Calreticulin precursor Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human, mouse, rat, primate, and chicken based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-Calreticulin precursor Antibody, rabbit anti-Calreticulin Antibody, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, Calreticulin precursor, CRTC, gpr60, CALR, grp60, HACBP, erp60

Purification: Anti-Calreticulin Precursor was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Calreticulin precursor affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human calreticulin precursor protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Calreticulin Precursor antibody has been tested in ELISA and western blot and is useful for immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.2 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.42 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR

Background: Background: Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response