DLD antibody (HRP)
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- Target See all DLD Antibodies
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
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Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DLD antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Cross reactivity against Lipoamide Dehydrogenase from other sources is unknown.
- Purification
- Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].
- Immunogen
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Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product DLD Primary Antibody
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- Application Notes
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Application Note: Anti-Lipoamide Dehydrogenase has been assayed against 1.0 μg of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- DLD (Dihydrolipoamide Dehydrogenase (DLD))
- Alternative Name
- Lipoamide Dehydrogenase (DLD Products)
- Synonyms
- DLDD antibody, DLDH antibody, E3 antibody, GCSL antibody, LAD antibody, PHE3 antibody, AI315664 antibody, AI746344 antibody, wu:fb24b05 antibody, DLD antibody, DDBDRAFT_0183800 antibody, DDBDRAFT_0216232 antibody, DDB_0183800 antibody, DDB_0216232 antibody, sc:d0402 antibody, dihydrolipoamide dehydrogenase antibody, dihydrolipoyl dehydrogenase antibody, deltaD antibody, DLD antibody, Dld antibody, dldh antibody, AT4G16155 antibody, CND05840 antibody, bfmBC antibody, GCSL antibody, LACBIDRAFT_182385 antibody, UREG_06178 antibody, lpd antibody, TAGG_RS02070 antibody, Arnit_2606 antibody, Mesil_1945 antibody, Trad_2118 antibody, Acear_0640 antibody, Fbal_0372 antibody, Ilyop_1890 antibody, Ftrac_1733 antibody, Ocepr_1753 antibody, Intca_2017 antibody, Deima_0504 antibody, dld antibody
- Background
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Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial EC=1.8.1.4 Dihydrolipoamide dehydrogenase Lipoamide Dehydrogenase [Porcine Heart]
Background: Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
Gene Name: DLD LAD
- Gene ID
- 397129
- UniProt
- P09623
- Pathways
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
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