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Dextranase antibody (HRP)

Reactivity: Penicillium sp. WB, ELISA, IP Host: Rabbit Polyclonal HRP
Catalog No. ABIN5596708
  • Target See all Dextranase products
    Dextranase
    Reactivity
    Penicillium sp.
    Host
    • 10
    Rabbit
    Clonality
    • 10
    Polyclonal
    Conjugate
    • 5
    • 4
    • 1
    This Dextranase antibody is conjugated to HRP
    Application
    • 9
    • 8
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against Dextranase from other sources is unknown.
    Purification
    Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].
    Immunogen

    Immunogen: Dextranase [Penicillium species]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-Dextranase has been assayed against 1.0 μg of Dextranase [Penicillium species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Dextranase
    Abstract
    Dextranase Products
    Synonyms
    dextranase antibody, Dextranase antibody, Teth39_0264 antibody, Teth514_0757 antibody, VDBG_04792 antibody, Thet_2161 antibody, Thebr_0271 antibody
    Background

    Synonyms: Dextranase Alpha-1,6-glucan-6-glucanohydrolase DEX

    Background: Dextranase belongs to the glycosyl hydrolase 49 family. It's catalytic reaction is endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.

    Gene Name: DEX

    UniProt
    P48845
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