HDAC4 antibody (AA 150-200)

Catalog No. ABIN5596723

Supplier Product No.: 600-401-j42

This anti-HDAC4 antibody is a Rabbit Polyclonal antibody detecting HDAC4 in WB and ELISA. Suitable for Human.

Quick Overview for HDAC4 antibody (AA 150-200) (ABIN5596723)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HDAC4 Antibody

Binding Specificity: AA 150-200

Supplier Product No.: 600-401-j42

Supplier: Rockland

Purpose: HDAC4 (internal) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-HDAC-4 antibody, HDAC4, HDAC 4, HD 4 antibody, histone deacetylase 4 antibody, Histone deacetylase-4, HD4 antibody

Purification: Anti-HDAC4 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 150-200aa of mouse HDAC4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HDAC4 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~140 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.26 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: HDAC4

Background: Background: HDAC4 antibody belongs to class II of the histone deacetylase/acuc/apha family. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC4 represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. Anti-HDAC4 is ideal for investigators interested in Stem Cell , Cardiovascular , and Epigenetics and Nuclear Signaling research.

Gene ID: 9759

NCBI Accession: NP_006028

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process