LMW Host Cell Proteins (LMW HCP) antibody

Details for Product No. ABIN5596744
Escherichia coli (E. coli)
ELISA, Western Blotting (WB)
Purpose Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli.

Immunogen: Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli.

Immunogen Type: Native Protein

Cross-Reactivity (Details) Reactivity is directed against low molecular weight E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, low molecular weight E.Coli HCP proteins antibody, LMW HCP antibody

Background: Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

Purification This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.
Alternative Name LMW E.Coli Host Cell Protein
Application Notes

Western Blot Dilution: 1:500 - 1:1000

ELISA Dilution: 1:1000-1:2000


Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and is suitable for western blotting and ELISA.

Restrictions For Research Use only
Format Liquid
Concentration 72.5 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store LMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-LMW Host Cell Proteins (LMW HCP) antibody (ABIN5596744) 2D Western Blot of anti- E.coli Low Molecular Weight Host Cell Protein antibody. Load...
Image no. 2 for anti-LMW Host Cell Proteins (LMW HCP) antibody (ABIN5596744) ELISA of Rabbit anti-Low Molecular Weight Host Cell Protein antibody. Antigen: BSA co...