RPS6KA1 antibody (C-Term)

Catalog No. ABIN5596747

Supplier Product No.: 600-401-941

This anti-RPS6KA1 antibody is a Rabbit Polyclonal antibody detecting RPS6KA1 in WB, ELISA and IP. Suitable for Mouse.

Quick Overview for RPS6KA1 antibody (C-Term) (ABIN5596747)

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-RPS6KA1 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-941

Supplier: Rockland

Purpose: p90 RSK1 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against the human RSK1 protein.

Characteristics: Synonyms: 90 kDa ribosomal protein S6 kinase 1 antibody, dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody, dJ590P13.1 antibody, EC 2.7.11.1 antibody, HU 1 antibody, RSK1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human RSK1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot, and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 88 kDa in size corresponding to RSK1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:1000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:2,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RPS6KA1

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Alternative Name: RPS6KA1

Background: Background: RSK1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsk's appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK1 is studied in cancer research and is known to inactive tumor suppressor complexes and death kinases.

Gene ID: 6195

NCBI Accession: NP_001006666

UniProt: Q15418

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades