Biotin antibody (Texas Red (TR))

Catalog No. ABIN95630

Supplier Product No.: 200-4998

The Texas Red (TR)-conjugated Rabbit Polyclonal anti-Biotin antibody (ABIN95630) specifically detects Biotin in WB, FACS and FM.

Quick Overview for Biotin antibody (Texas Red (TR)) (ABIN95630)

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to Texas Red (TR)

Application: Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Product Details anti-Biotin Antibody (Texas Red (TR))

Supplier Product No.: 200-4998

Supplier: Rockland

Purpose: Biotin Antibody Texas Red™ Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.

Characteristics: Synonyms: rabbit anti biotin Antibody Texas Red™ Conjugated, rabbit anti-biotin Texas Red ™ conjugated antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-Biotin Antibody Texas Red Conjugate has been tested by western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

IF Microscopy Dilution: 1:500-1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Texas Red Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.