Phosphotyrosine antibody (Biotin)

Catalog No. ABIN5596786

Supplier Product No.: 600-406-262

The Biotin-conjugated Rabbit Polyclonal anti-Phosphotyrosine antibody (ABIN5596786) specifically detects Phosphotyrosine in WB, IP, FACS, IHC and ELISA.

Quick Overview for Phosphotyrosine antibody (Biotin) (ABIN5596786)

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphotyrosine antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Product Details anti-Phosphotyrosine Antibody (Biotin)

Supplier Product No.: 600-406-262

Supplier: Rockland

Purpose: Phosphotyrosine Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

Characteristics: Synonyms: PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit

Purification: Anti-PhosphoTyrosine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-PhosphoTyrosine was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-PhosphoTyrosine antibody is tested for use in ELISA, immunohistochemistry-P, IP, and western blotting assays.  The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues.  The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA and flow cytometry.

Western Blot Dilution: 1:1,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-pY at -20° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Background: Anti-PhosphoTyrosine detects phosphorylated Tyrosines. Tyrosine is one of the 22 amino acids that are used by cells to synthesize proteins. Tyrosine has a phenol group. It functions as a receiver of phosphate groups that are transferred by way of protein kinases. Phosphorylation of the hydroxyl group changes the activity of the target protein. Anti-PhosphoTyrosine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.