Phosphotyrosine antibody (HRP)

Catalog No. ABIN5596787

Supplier Product No.: 600-403-262

This Rabbit Polyclonal antibody specifically detects Phosphotyrosine in WB, IP, IHC and ELISA.

Quick Overview for Phosphotyrosine antibody (HRP) (ABIN5596787)

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphotyrosine antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA

Product Details anti-Phosphotyrosine Antibody (HRP)

Supplier Product No.: 600-403-262

Supplier: Rockland

Purpose: Phosphotyrosine Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Peroxidase.

Characteristics: Synonyms: HRP Anti-Phospho-Tyrosine, PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity-purified antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues.  The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:1,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Background: The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression.