PIPOX antibody (HRP)

Catalog No. ABIN5596798

Product Details anti-PIPOX Antibody (HRP)

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Reactivity: Microbial

Host: Goat

Clonality: Polyclonal

Conjugate: This PIPOX antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Sarcosine Oxidase from other sources is unknown.

Purification: Anti-Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].

Immunogen:

Immunogen: Sarcosine Oxidase [Microbial]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Sarcosine Oxidase has been assayed against 1.0 μg of Sarcosine Oxidase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PIPOX

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Alternative Name: Sarcosine Oxidase (PIPOX Products)

Background:

Synonyms: Sarcosine oxidase subunit alpha Sarcosine oxidase subunit EC=1.5.3.1

Background: Sarcosine oxidase is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide, and 5,10-methylenetetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.

Gene Name: SOXA

Gene ID: 10586229

UniProt: Q46337