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APOA1 antibody

APOA1 Reactivity: Human WB, ELISA, IHC, IP Host: Goat Polyclonal unconjugated
Catalog No. ABIN5596807
  • Target See all APOA1 Antibodies
    APOA1 (Apolipoprotein A-I (APOA1))
    Reactivity
    • 119
    • 39
    • 23
    • 10
    • 10
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 91
    • 51
    • 17
    • 5
    • 3
    • 3
    • 2
    Goat
    Clonality
    • 112
    • 58
    Polyclonal
    Conjugate
    • 86
    • 33
    • 15
    • 13
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This APOA1 antibody is un-conjugated
    Application
    • 108
    • 81
    • 42
    • 21
    • 19
    • 15
    • 13
    • 13
    • 10
    • 8
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
    Purification
    This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
    Immunogen

    Immunogen: apoLipoprotein Type A-I was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

    Immunogen Type: Native Protein

    Isotype
    IgG
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    Discover our top product APOA1 Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation, immunohistochemistry and for western blotting for highly sensitive qualitative analysis.

    Western Blot Dilution: 1:1,000 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:10,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Jani, Orlicky, Karimpour-Fard, Epperson, Russell, Hunter, Martin: "Kidney proteome changes provide evidence for a dynamic metabolism and regional redistribution of plasma proteins during torpor-arousal cycles of hibernation." in: Physiological genomics, Vol. 44, Issue 14, pp. 717-27, (2012) (PubMed).

    Otis, Sahoo, Drover, Yen, Carey: "Cholesterol and lipoprotein dynamics in a hibernating mammal." in: PLoS ONE, Vol. 6, Issue 12, pp. e29111, (2012) (PubMed).

    Martin, Epperson, Rose, Kurtz, Ané, Carey: "Proteomic analysis of the winter-protected phenotype of hibernating ground squirrel intestine." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 295, Issue 1, pp. R316-28, (2008) (PubMed).

  • Target
    APOA1 (Apolipoprotein A-I (APOA1))
    Alternative Name
    APOLIPOPROTEIN A-I (APOA1 Products)
    Synonyms
    Alp-1 antibody, Apoa-1 antibody, Brp-14 antibody, Ltw-1 antibody, Lvtw-1 antibody, Sep-1 antibody, Sep-2 antibody, Sep2 antibody, apoA-I antibody, apoa antibody, apoa1 antibody, cb49 antibody, wu:fb33f01 antibody, zgc:103718 antibody, MGC64335 antibody, MGC89745 antibody, Apo-AI antibody, ApoA-I antibody, APOA1 antibody, LOC100136573 antibody, apoa-i antibody, ApoAI antibody, apolipoprotein A1 antibody, apolipoprotein A-I antibody, apolipoprotein A-Ia antibody, apolipoprotein A-I S homeolog antibody, apolipoprotein A4 antibody, APOA1 antibody, Apoa1 antibody, apoa1a antibody, apoa1.S antibody, apoa1 antibody, LOC100136573 antibody, apoa-i antibody, apoa4 antibody
    Background

    Synonyms: APOA1 antibody, APOA1/APOC3 fusion gene antibody, Apolipoprotein A I precursor antibody, Apolipoprotein AI antibody, Apolipoprotein of high density lipoprotein antibody

    Background: Anti Apolipoprotein A-I antibody recognizes the gene product of APOA1. Apolipoprotein promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease. This antibody is suitable for cardiovascular research.

    Gene Name: APOA1

    Gene ID
    335
    UniProt
    P02647
    Pathways
    Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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