Phosphothreonine antibody

Catalog No. ABIN5596876

Supplier Product No.: 600-401-263

The Rabbit Polyclonal anti-Phosphothreonine antibody (ABIN5596876) specifically detects Phosphothreonine in WB, IP, ELISA and IHC. The antibody is reactive with Mouse samples.

Quick Overview for Phosphothreonine antibody (ABIN5596876)

Target: Phosphothreonine

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphothreonine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)

Product Details anti-Phosphothreonine Antibody

Supplier Product No.: 600-401-263

Supplier: Rockland

Purpose: Phosphothreonine Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.

Characteristics: Synonyms: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit

Purification: Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose.

Sterility: Sterile filtered

Immunogen:

Immunogen: PhosphoThreonine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity-purified antibody is tested for use in ELISA, western blotting and immunoprecipitation assays.  The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues.  The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 2.0 μg/mL

Immunoprecipitation Dilution: 10.0 μg/mg sample

ELISA Dilution: 0.5 μg/mL

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Phosphothreonine

Target: Phosphothreonine

Target Type: Amino Acid

Background: Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.