XDH antibody (HRP)

Catalog No. ABIN5596882

Supplier Product No.: 200-4383-0100

This anti-XDH antibody is a Rabbit Polyclonal antibody detecting XDH in WB, IP and DB. Suitable for Cow.

Quick Overview for XDH antibody (HRP) (ABIN5596882)

Target: XDH (Xanthine Dehydrogenase (XDH))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Product Details anti-XDH Antibody (HRP)

Supplier Product No.: 200-4383-0100

Supplier: Rockland

Purpose: Xanthine Oxidase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Characteristics: Synonyms: rabbit anti-Xanthine Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

Purification: Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Xanthine Oxidase [Bovine Buttermilk]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Xanthine Oxidase Peroxidase conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250

Immunoprecipitation Dilution: 1:100

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: XDH

Background: Background: Xanthine Oxidase is a key enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine, xanthine to uric acid, and contributes to the generation of reactive oxygen species.

Gene ID: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity