CA1 antibody (HRP)

Catalog No. ABIN5596907

Product Details anti-CA1 Antibody (HRP)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CA1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined.

Purification: Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ].

Immunogen:

Immunogen: Carbonic Anhydrase I [Human Erythrocytes ]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Carbonic Anhydrase I is useful in ELISA, western blot, and Immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: Carbonic Anhydrase I (CA1 Products)

Synonyms: CA-I antibody, CAB antibody, Car1 antibody, AW555628 antibody, Ca1 antibody, Car-1 antibody, BG:DS00941.1 antibody, CAH antibody, CG7820 antibody, Dmel\\CG7820 antibody, Gh7 antibody, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 antibody, ATBCA1 antibody, ATSABP3 antibody, BETA CARBONIC ANHYDRASE 1 antibody, F4P13.5 antibody, F4P13_5 antibody, SABP3 antibody, SALICYLIC ACID-BINDING PROTEIN 3 antibody, carbonic anhydrase 1 antibody, CA1 antibody, LOC100135826 antibody, GB15888 antibody, carbonic anhydrase 1 antibody, Carbonic anhydrase 1 antibody, carbonic anhydrase I antibody, carbonic anhydrase antibody, CA1 antibody, Car1 antibody, CAH1 antibody, LOC100135826 antibody, LOC408827 antibody, Tsp_05114 antibody, LOC100153915 antibody, LOC100050192 antibody, Ca1 antibody

Background:

Synonyms: Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

Background: Carbonic Anhydrase I is an enzyme that in humans is encoded by the CA1 gene. Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.

Gene Name: CA1

Gene ID: 759

UniProt: P00915