Trypsin Inhibitor antibody (HRP)
-
- Target See all Trypsin Inhibitor products
- Trypsin Inhibitor
-
Reactivity
- Lima bean
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Trypsin Inhibitor antibody is conjugated to HRP
-
Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.
- Purification
- Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].
- Immunogen
-
Immunogen: Trypsin Inhibitor [Lima Bean]
Immunogen Type: Native Protein
- Isotype
- IgG
-
-
- Application Notes
-
Application Note: Anti-Trypsin Inhibitor has been assayed against 1.0 μg of Trypsin Inhibitor [Lima Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:40,000
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- Trypsin Inhibitor
- Abstract
- Trypsin Inhibitor Products
- Synonyms
- CRISP11 antibody, LCRISP2 antibody, GRMZM2G447785 antibody, cysteine rich secretory protein LCCL domain containing 2 antibody, trypsin inhibitor antibody, trophoblast Kunitz domain protein 1 antibody, Kazal-type serine proteinase inhibitor 1 antibody, Bowman-Birk type bran trypsin inhibitor antibody, CRISPLD2 antibody, LOC548021 antibody, TKDP1 antibody, Spi1 antibody, LOC100284348 antibody
- Background
-
Synonyms: 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
Background: Trypsin Inhibitors are chemicals that reduce the availability of biologically active trypsin, an enzyme essential to nutrition of many animals, including humans. Tumor Associated Trypsin Inhibitor (TATI) has been used as a marker for various carcinomas and useful in cancer diagnosis.
Gene Name: KTI3
- Gene ID
- 547831
- UniProt
- P01070
-