SETDB2 antibody

Catalog No. ABIN5596921

Supplier Product No.: 200-401-n99

This anti-SETDB2 antibody is a Rabbit Polyclonal antibody detecting SETDB2 in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for SETDB2 antibody (ABIN5596921)

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SETDB2 Antibody

Supplier Product No.: 200-401-n99

Supplier: Rockland

Purpose: Setdb2 Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against mouse Setdb2.

Characteristics: Synonyms: rabbit anti-Setdb2, Histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, Setdb 2 antibody, Gm293

Purification: The product was purified from monospecific antiserum by Protein A affinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Setdb2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length protein corresponding to mouse Setdb2.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Setdb2 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80.6 kDa in size corresponding to Setdb2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:10,000 - 1:100,000

ELISA Dilution: 1:2,500 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SETDB2

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Alternative Name: Setdb2

Background: Background: Setdb2 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). Setdb2 and the neighboring gene Phf11 were linked to familial atopy/ asthma and are in a "critical region" commonly deleted in CLL. Setdb2 is a candidate histone methyltransferase as a member of the Su(var) 3-9 family of SET domain-containing enzymes. Other predicted domains in Setdb2 include a methylcytosine binding domain and a large bifurcation domain interrupting the putative histone methyltransferase (SET) domain whose function is unknown. Anti-Setdb2 antibody is ideal for researchers interested in Cancer Research and Epigenetics Research.

UniProt: Q8C267