FOLR1 antibody (HRP)

Catalog No. ABIN5596925

Supplier Product No.: 200-103-148-0100

This Goat Polyclonal antibody specifically detects FOLR1 in WB, ELISA and IP. It exhibits reactivity toward Cow.

Quick Overview for FOLR1 antibody (HRP) (ABIN5596925)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This FOLR1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-FOLR1 Antibody (HRP)

Supplier Product No.: 200-103-148-0100

Supplier: Rockland

Purpose: Folate Binding Protein Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk].

Characteristics: Synonyms: goat anti-Folate Binding Protein Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Folate Binding Protein Antibody, Folate receptor alpha, FR-alpha, Folate receptor 1, Milk folate-binding protein, Folate-binding protein 1, FBP

Purification: Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Folate Binding Protein [Bovine Milk]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Folate Binding Protein has been tested in western blot and is suitable to be assayed against 1.0 μg of Folate Binding Protein [Bovine Milk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: FOLR1

Background: Background: Folate-binding protein (FBP) are proteins that bind folate, typically the folate receptors and reduced folate carrier. In cancer research it can be a marker for ovarian cancer. It can be measured to help in tumor classification to aid treatment. FBP is over-expressed (20-80 fold) in more than 90 % of ovarian and endometrial cancers, as well as 20-50 % of breast, lung, colorectal, and renal cell carcinomas. FBP has very limited tissue distribution and expression in non-malignant tissue, making it a good immunotherapy target.

Gene ID: 539750

NCBI Accession: NP_001193456

UniProt: P02702

Pathways: Dicarboxylic Acid Transport