Protein A antibody (Texas Red (TR))

Catalog No. ABIN5596971

Product Details anti-Protein A Antibody (Texas Red (TR))

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Goat

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to Texas Red (TR)

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Purification: Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum.

Immunogen:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 1.5

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-Protein A Texa Red Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

IF Microscopy Dilution: 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Protein A

Target: Protein A

Abstract: Protein A Products

Synonyms: alpha protein antibody, repB antibody

Background:

Synonyms: Texas Red™, Protein A Texas Red™ Antibody

Background: Anti-Protein A Texas Red is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.